Feb 13, 2023 · Hydrophobic interaction chromatography (HIC) is a column chromatographic separation method that is commonly used to purify and isolate macromolecules such as proteins and …

Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. HIC is a useful separation technique for purifying proteins while maintaining biological activity due to …

Hydrophobic interaction chromatography (HIC) is a chromatographic technique that mainly targets the separation of biomolecules (intact proteins, monoclonal antibodies, etc.) based on the difference …

Cytiva introduced the first commercial HIC media in 1977 and now offers one of the broadest ranges of HIC media on the market, covering most common laboratory and industrial applications.

Hydrophobic interaction chromatography (HIC) is a liquid chromatography to separate and purify biomolecules by their hydrophobic interaction with the hydrophobic ligands coupled to porous media. …

Hydrophobic interaction chromatography involves the separation of molecules based on their hydrophobicity. HIC exploits the interaction between hydrophobic molecules in a sample and …

Oct 24, 2025 · What is Hydrophobic Interaction Chromatography (HIC)? HIC is a chromatographic technique used for the separation and purification of biomolecules, particularly proteins, based on …

Hydrophobic interaction chromatography (HIC) is used in bioprocessing as a polishing step to improve purity and resolution by exploiting differences in hydrophobicity between the target molecule …

Hydrophobic interaction chromatography (HIC) separates biomolecules, under comparatively mild conditions, according to differences in hydrophobicity on the exposed surface of the molecule.

This blog series will tell you everything you need to know (I hope!) about a mode of chromatography that you might not be familiar with: hydrophobic interaction chromatography (HIC).